1	"Megan Westall" Megan Westall October 30, 2003
2	Veterinary Diagnostic Center VDC Basics Internship Essentials Laboratory Marketing/Research Leasons Learned
3	VDC Basics Subsidiary of DNA Diagnostics Center (DDC) Located in Fairfield, OH World’s largest private laboratory for human DNA testing Opened 1994 recommended by more hospitals and physicians than all other laboratories combined Testing performed in all 50 states and over 73 countries Performs DNA parentage testing for Maury Povich, Ricki Lake, Montel Williams, Primetime Live, & Judge Hatchett
4	VDC Basics Cont. Matches DDC’s quality and integrity Every Test is Run Twice Lowest Prices Fastest Turnaround Members of ISAG Reliable Results Combined over 75 years in DNA technology, paternity testing and cytogenetic studies
5	VDC Basics Cont. Started Canine Testing Summer 2003 Parentage Testing Inherited Disease Screening DNA Banking DNA Profiling Validating equine testing
6	Internship Essentials History with the Company Worked over breaks for the past 3 years Kit Prep/Case Info Dates: June 18, 2003 – September 12, 2003 Time Split Lab Marketing/Research
7	Laboratory 40-50 Hours Laboratory In Service Using a Micro Pipette DNA Extraction Lysing Solution Heating Neutralizing Solution
8	Laboratory Cont. PCR Procedure Thermal Cycler Heated to denature Cooled to allow the primers to work Heated again for replication Template DNA Helicase & Gyrase Primers DNA Polymerase
9	Laboratory Cont. Analyzing Data ABI-3100 Capillary Electrophoresis
10	Marketing/Research Web Research Contacts Found and Cataloged “American Kennel Club” links 187 Websites Based on AKC breed list Breed Clubs 1875 Websites Breeders 4030 Websites Only completed through 23 out of 150
11	Marketing/Research Cont. Horse Registries DNA/Blood typing requirements Costs of DNA/Blood Typing Total number registered Amount registered per year Who performs their tests currently
12	Marketing/Research Cont. Blood Typing vs. DNA Blood Typing Past Small Registries Haven’t changed due mostly to cost DNA Up to Date More Accurate
13	Marketing/Research Cont. Canine Collection Coordinated collection of 70 employee samples Database calculations
14	Marketing/Research Cont. Horse Collection For Validation Collected over 100 samples My 2 horses OSU Equine Center Old Stone Riding Center Family/Friends Cheryl Quick in Chicago Other Employees
15	Marketing/Research Cont. Start to Finish Made collection procedures Coordinated where to collect Created sample kits Recorded Owner/Horse Information Return to help with testing Collected Hair Buccal Swabs
16	Odds & Ends Blood Typing vs. DNA Interview Meetings Horse Knowledge Company Formings Brochure Creation Certificate Details
17	Marketing/Research Cont.
18	Lessons Learned Laboratory Safety Micropipette DNA Extraction PCR Mechanics Actual Process Capillary Electrophoresis ABI 3100 DNA Analysis
19	Lessons Learned Cont. Marketing Initial Research Web Competitors Collection Procedures Coordination with Contacts Veterinarians Breeders Researchers Registries
20	"Dr." Dr. Richard & Dr. Susanne Lee Randy Smith David Ingala Questions???